Home / Clinical Laboratory / Chemicals and Solutions / Immunology Reagent / Immunology Reagent BD Phosflow™ Lysis Buffer Flow Cytometry 5X 250 mLBDB558049
Picture of Immunology Reagent BD Phosflow™ Lysis Buffer Flow Cytometry 5X 250 mLBDB558049

Immunology Reagent BD Phosflow™ Lysis Buffer Flow Cytometry 5X 250 mLBDB558049

Immunology-Reagent-BD-Phosflow™-Lysis-Buffer-Flow-Cytometry-5X-250-mLBDB558049

SKU #

#866831

MPN #

BDB558049

Brand

Fisher Scientific

Stock

In Stock

Quantity

100

Features

BUFFER, BD PHOSFLOW LYSE FIX 250ML

$329.27 USD

Product Specifications Product Usage Additional Information
Print
Attribute Name Attribute Value
McKesson # 866831
Manufacturer # BDB558049
Brand BD Phosflow™
Manufacturer Fisher Scientific
Application Immunology Reagent
Chemical Name Lysis Buffer
Concentration Level 5X
Container Type Amber Poly Bottle
For Use With For use with Flow Cytometer
Form Aqueous Solution
Type Flow Cytometry
UNSPSC Code 41116127
Volume 250 mL
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  • BD 558049 - can be used for lysing red blood cells and for fixing leukocytes in one step, allowing investigators to rapidly inactivate kinases, phosphatases and/or proteases, so that the in vivo phosphorylation state of the cell can be examined
  • BD Phosflow™ Lyse/Fix Buffer is intended to be used for the lysing and fixing of whole blood for use with intracellular flow cytometry
  • Has been reported to preserve the light scattering properties of the cell
  • May be used on human and mouse whole blood
  • Whole blood lysis has been shown to be as effective as density gradient centrifugation in the preparation of PBMCs for lymphocyte subset analysis
  • In many cases, phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g. CD markers); however, not all cell surface markers, however, are compatible with the BD Phosflow Lyse/Fix buffer